Artificial insemination (AI) procedure

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Source:

https://www.youtube.com/watch?v=KkT79NAw6kc

Duration: 

00:13:46

Year of Production: 

2016

Source/Author: 

Digital Agriculture
»This video consists of brief introduction about importance of AI, materials required, semen straw identification and proper handling, thawing procedure, loading of semen straws in AI gun, preparation of oestrous animal, proper insemination procedure and clitoris massaging for better conception.«

Artificial Insemination (AI) is the process by which the semen is deposited inside the reproductive tract of the female by means of instruments.

AI helps to propagate superior germ plasm and prevents spread of sexually transmitted diseases. There are several materials needed like liquid nitrogen tin, frozen semen straws, long forceps pipe, scissors, thermometer, warm water. Additionally ensure no gap between straw end cut and the sheath during the process to avoid part of the semen from remaining in the sheath. Oestrus should be determined by clinical or gynaecological examination.

Steps to follow

Start by identifying the bull number from the tag attached on canister, then lift the concerted canister up to the neck, pick the straw with forceps as fast as possible and shape them to remove excess nitrogen.

Thereafter strictly follow temperature and time when putting the straw in warm water to achieve high semen recovery rate.

After thawing gently wipe straws to remove water however, water should be lethal to spermatozoa.

Then slide sheet over the gun and tightly secure the gun with 0- ring. Ensure wearing protective gloves, lubricants and properly widen perineum and valval area to avoid infection on the gun.

Additionally, open the valve eclipse and insert the gun at 30- 40 angle to avoid urethral opening. Pass the gun gently and fix it at the external walls of the cervix, slowly guide it into the cervix till it reaches the internal uterus walls.

After AI Massage the clitoris ten times to get favourable results and lastly insemination should be done at proper oestrus times to obtain high conception rate.

Sequence from Sequence to Description
00:0000:48AI is used to propagate superior germ plasm and prevention of sexually transmitted diseases.
00:4903:10Materials: liquid nitrogen tin, frozen semen straws, long forceps pipe, scissors, thermometer and warm water.
03:1103:56Identify the bull number, lift the concerted canister to the neck and pick the straw.
03:5705:27Pick straws from containers with forceps faster and shape them.
05:2806:49Strictly following temperature and time immediately put the straw in warm water.
06:5007:16After thawing gently wipe straws. Water should be lethal to spermatozoa.
07:1707:44Gently place straws into the gun and cut at laboratory seal.
07:4508:09Slide sheet over the gun and tightly secure the gun with 0- ring.
08:1008:35Ensure no gap between straw end cut and the sheath.
08:3609:32For insemination use protective gloves and lubricants and properly widen perineum and valval area.
09:3310:43Open the valve eclipse and insert the gun.
10:4411:28Pass the gun gently and fix it at external cervix walls, guide into the cervix till the internal uterus walls.
11:2911:56When gun reaches internal uterus walls slowly deposit semen by steel pushing.
11:5712:48After AI, massage the clitoris ten times and inseminate at proper oestrus.
12:4913:08Clear mucus discharge from vulva is good oestrus indicator for insemination,
13:0913:46Inseminate the same day of oestrus signs shows up.

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